Linked Life Data

36994

Source:http://linkedlifedata.com/resource/pubmed/id/36994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1979-9-27
pubmed:abstractText
Light scattering measurements were used to monitor the integrity of isolated rat kidney lysosomes during prolonged incubation at 37 degrees C or following the addition of lysolecithin. The fall in extinction at 520 nm (E520) was shown to correlate very well with the fall in the particulate enzyme activity and the corresponding rise in the soluble enzyme activity. Measurements were also made of the release of H+ from the lysosomes into the suspending medium following treatment with lysolecithin. A good relationship was obtained between acidification of the medium and changes in the light scattering (E520) of the lysosomal suspension. The value of these techniques in following rapid changes in the integrity of lysosomes is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0009-2797
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
The monitoring of lysosomal integrity by pH-stat and light scattering measurements.
pubmed:publicationType
Journal Article, In Vitro