Linked Life Data

369848

Source:http://linkedlifedata.com/resource/pubmed/id/369848

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1979-5-26
pubmed:abstractText
The relationship between beta-cell replication and insulin release was investigated utilizing neonatal rat pancreatic monolayer cell cultures. Glucose-induced insulin release was either inhibited using diazoxide or mannoheptulose, or potentiated using theophylline. The corresponding effects on the frequency of beta-cell replication were determined by incubating cultures with [3H]thymidine and estimating the frequency of beta-cell labeling in aldehyde thionine-stained radioautographs. beta-Cell replication and insulin release were shown to be dissociable processes in two ways. First, in the presence of diazoxide (1-100 microgram/ml), insulin release was inhibited by as much as 86%, while the frequency of beta-cell replication was not reduced. Second, in the presence of theophylline (1 mM), insulin release was increased by 23%, while beta-cell replication was inhibited. Finally, the inhibition of both beta-cell replication and insulin release by mannoheptulose (5.5 mM) indicated that glucose utilization may be important for both of these beta-cell processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
103
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1321-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Pancreatic beta-cell replication: relation to insulin secretion.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.