Linked Life Data

369603

Source:http://linkedlifedata.com/resource/pubmed/id/369603

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-5-26
pubmed:abstractText
The sequences of two promoters recognized by the phage-specificied T7 RNA polymerase are presented. The two are identical in sequence but for one base pair from the initiation point (as determined by the 5' sequence of the transcripts), denoted +1, to position -15. The common : formula: (see text), sequence also includes a region of hyphenated twofold symmetry indicated by the boxes, with the twofold axis as the center of the six base-pair box. The heavy line indicates the extent of homology. The first promoter (A) is demonstrated to lie within gene 1, the gene for the polymerase itself, and 40 bases into the message transcribed from this promoter is found the R Nase III site separating genes 1 and 1.1. Binding of T7 RNA polymerase to these promoters is associated with a hyperchromic blue shift of the base chromophores consistent with partial melting of the base pairs at the promoter. Binding of T7 RNA polymerase to these promoters disappears at low pH and low temperature and is accompanied by a consequent loss of polymerase activity. The pH dependence of the binding step is adequately described by a single pK of 7.0. Polymerase catalytic activity, but not promoter binding, requires a single free sulfhydryl group of the enzyme with a pKa of approximately 7.8.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
528-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
T7 RNA polymerase: promoter structure and polymerase binding.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.