Linked Life Data

369467

Source:http://linkedlifedata.com/resource/pubmed/id/369467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1979-4-25
pubmed:abstractText
The reactivity of F(ab)2 fragments of sperm antibodies was studied in agglutination, immobilization and immunofluorescence tests. Pepsin digestion of full serum was an easy and effective procedure for preparing F(ab)2 fragments. Compared to the reactions with serum, F(ab)2 preparations revealed only a moderate decrease in agglutination titres but a complete loss of immobilizing activity. In immunofluorescence tests, nonspecific background staining was strongly reduced by using F(ab)2 fragments, thereby offering a more reliable basis for the reading of reactions. Even though the titres obtained with serum and F(ab)2 preparations did not differ essentially, it is suggested that the use of F(ab)2 fragments in IF-tests on spermatozoa may help to provide a higher degree of reproducibility.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0148-5016
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-8
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Reactivity of F(ab)2 fragments of sperm antibodies in agglutination, immobilization and immunofluorescence tests.
pubmed:publicationType
Journal Article