Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-2-8
pubmed:abstractText
The use of a novel amide surfactant, N-methyl oleoyl taurate (Igepon T-77), has been examined for the separation of amines on reversed-phase chromatographic material. This reagent was found to partition onto the C18 material in a partially irreversible and concentration independent manner. When the stationary phase is saturated with this surfactant, the loaded column performs as a strong cation exchanger. Novel separations are possible as a result of secondary hydrogen-bonding effects which modify classical retention order for primary, secondary and tertiary amines. Sensitive and selective applications of these separations are demonstrated for catecholamine determinations in blood plasma, cerebrospinal fluid, urine and brain tissue. Additional sensitivity is obtained for epinephrine by taking advantage of the pH-dependent intramolecular cyclization and on-column concentration of large injection volumes.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
420
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-51
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Application of a novel cation-exchange reagent, Igepon T-77 (N-methyl oleoyl taurate), to microbore separations of alumina extracts of catecholamines from cerebrospinal fluid, plasma, urine and brain tissue with amperometric detection.
pubmed:affiliation
Section on Clinical Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article