Linked Life Data

3690894

Source:http://linkedlifedata.com/resource/pubmed/id/3690894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-1-25
pubmed:abstractText
Using one-dimensional and two-dimensional immunoblotting techniques the reactions of sera from 14 patients with liver-kidney microsomal (LKM) antibody positive chronic active hepatitis (CAH) with human liver microsomal preparations was compared with the reaction of sera from 12 healthy persons and from five patients with systemic lupus erythematosus (SLE). All sera displayed a multiplicity of reactions. This demonstrates the presence of many autoantibodies in normal human sera. It could be shown that all sera react with the cytoskeletal antigens cytokeratin, actin and actomyosin. These reactions were more marked in the autoimmune sera, i.e. LKM-positive CAH and SLE. Densitometric subtraction was found to be a reliable technique to distinguish the natural antigen/autoantibody reactions from pathological, disease-characteristic autoantibodies. It was shown that the pathological LKM autoantibodies recognize non species-specific microsomal proteins at 50 kD of pI 7.5-8.0 at high titres, which are only very weakly recognized by normal or SLE sera. We recommend sensitive immunoblotting techniques and densitometric subtraction as the currently most accurate method to distinguish natural from pathological autoantibodies.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-13249955, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-3760567, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-3989471, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-4596402, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-4912327, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-6176652, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-6467682, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-6840686, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-7026097, http://linkedlifedata.com/resource/pubmed/commentcorrection/3690894-7047576
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Distinction between natural and pathological autoantibodies by immunoblotting and densitometric subtraction: liver-kidney microsomal antibody (LKM) positive sera identify multiple antigens in human liver tissue.
pubmed:affiliation
I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität Mainz, FRG.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't