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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-1-5
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pubmed:abstractText |
mRNAs from reticulocyte polysomes were fractionated by chromatography on poly(U)-Sepharose and thermal elution. The molar ratio of alpha- to beta-globin mRNA was found to be 2:1 and 1:1 respectively in short- and long-poly(A) size classes. Translational analyses indicated that the globin mRNAs containing long poly(A) tracts (with a mean length of about 70 nucleotides) directed protein synthesis with higher rates than did mRNA containing short poly(A) tracts (15-35 nucleotides). Experiments performed with sub-saturating mRNA concentrations showed that the digestion with RNAase H induced a decrease in the translational capacity of both globin mRNAs and an increase in the alpha- to beta-globin synthesis ratio. No correlation was observed between the size of the poly(A) tail in mRNA and the optimal K+ requirement for translation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-1148210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-269388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-2864691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-2878361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-3863133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4084510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4129862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-439140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4421673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4421725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4531006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4613842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-476057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-4835729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-558522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-558827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-592398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6089873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6101207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6137807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6142768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6159806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6165016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6271760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6274873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-646104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6498199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6539594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6572361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-6854649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-699050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-7259786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-7298630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-7441754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-776610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-856800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-90045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3689323-954092
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
246
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
575-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3689323-Base Sequence,
pubmed-meshheading:3689323-Chromatography, Affinity,
pubmed-meshheading:3689323-DNA,
pubmed-meshheading:3689323-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3689323-Globins,
pubmed-meshheading:3689323-Molecular Sequence Data,
pubmed-meshheading:3689323-Poly A,
pubmed-meshheading:3689323-Potassium,
pubmed-meshheading:3689323-Protein Biosynthesis,
pubmed-meshheading:3689323-RNA, Messenger
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pubmed:year |
1987
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pubmed:articleTitle |
Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs.
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pubmed:affiliation |
Departament de Microbiologia, Facultat de Farmacia, Universitat de València, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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