Linked Life Data

3687435

Source:http://linkedlifedata.com/resource/pubmed/id/3687435

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-12-30
pubmed:abstractText
The topographical distribution and characteristics of an apocrine epithelial differentiation antigen (AEA) were studied in one endometrial adenocarcinoma cell line (HEC-1-B) and two mammary carcinoma cell lines (MCF-7 and T47-D), using an antiserum raised against glycoproteins which had been isolated from human milk fat globule membranes. Immunofluorescent staining of HEC-1-B and MCF-7 cells grown in monolayers, or of histological sections of cells grown in fibrin sponges, revealed a strictly polar distribution of the antigen. The antigen was present only in the dorsal-apical cell membrane. In contrast, T47-D cells grown under identical conditions displayed a nonpolar membrane distribution of the antigen. Detergent lysates of HEC-1-B, MCF-7 and T47-D cells, surface-labeled by the PI(sodiumetaperiodate)-NaB3H4 method, were immunoprecipitated and analysed by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Two closely spaced bands having an apparent MW of 315-290 kD (kilodalton) (HEC-I-B), 330-295 kD (MCF-7) and 320-270 kD (T47-D). The surface expression of the antigen was found to be hormonally regulated. Cultivation of HEC-1-B and MCF-7 in the presence of prolactin increased the amount of antigen. The T47-D cells responded only weakly to prolactin, but displayed enhanced antigen expression after treatment by estrogen and/or progesterone, as quantified by 125I protein A radioimmunoassay. The behaviour of the membrane antigen reported here provides a new and interesting marker for the differentiation and maintenance of polarity in cultured malignant cells of secretory epithelial origin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0108-0164
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-23
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
An apocrine membrane antigen with polarized distribution and hormonally regulated expression in human endometrial and mammary carcinoma cell lines.
pubmed:affiliation
Department of Pathology, University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't