Linked Life Data

3681409

Source:http://linkedlifedata.com/resource/pubmed/id/3681409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-1-19
pubmed:abstractText
In addition to paired helical filaments (PHF), the Alzheimer neurofibrillary tangle (NFT) contains straight filaments measuring 10-15 nm in width, as well as amorphous material. In previous immunoelectron microscopic studies, we have demonstrated that PHF and the amorphous material share antigens with the microtubule associated protein, tau, and the 200 kDa subunit of neurofilaments (NF). The present immunoelectron microscopic study focuses on the antigens present in the straight filaments contained in the NFT. Indirect and direct immunogold staining of Vibratome sections of Alzheimer hippocampus with a monoclonal antibody to NF200 kDa (1.1.1.) and polyclonal antibodies to NF, tau, and to PHF yielded intense and specific reaction with NFT. Within the same NFT both straight filaments and PHF were decorated by all the antibodies. Similarly decorated within NFT were filaments displaying a transitional form between straight filaments and PHF. Both PHF and straight filaments were also recognized by all the antibodies after extraction with an ionic detergent. These findings show that straight filaments contain most, if not all, of the antigens known to be present in PHF and share with PHF insolubility in ionic detergents. It is suggested that straight filaments result from alternative pathways of organization of the same components as form the PHF.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3736-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Immunocytochemical properties of Alzheimer straight filaments.
pubmed:affiliation
Division of Neuropathology, Case Western Reserve University, Cleveland, Ohio 44106.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't