Linked Life Data

3680408

Source:http://linkedlifedata.com/resource/pubmed/id/3680408

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1988-1-15
pubmed:abstractText
The Stokes radius of the rat liver glucocorticoid receptor was determined using TSK G3000SW and TSK G4000SW high-performance size-exclusion columns. The accuracy of the calibration graph for proteins larger than 6 nm on the TSK G4000SW column allowed the resolution of a heterogeneous structure for the cytosolic untransformed receptor, giving two forms with Rs values of 8.3 and 7.1 nm, whereas the transformed receptor elutes with an Rs value of 4.7-5.3 nm. The 8.3 nm form was not observed for the highly purified untransformed receptor. Parallel analyses of the cytosolic untransformed receptor on conventional gravity-fed Bio-Gel A 1.5-m or Ultrogel AcA-22 size-exclusion columns could not resolve two components. The resolution efficiencies of high-performance size-exclusion chromatography and open-column size-exclusion chromatography were compared. Further, owing to its rapidity, high-performance chromatography allowed the characterization of steroid-receptor complexes having half-lives as short as 90 min and very unstable receptor forms could be detected. Specific applications are considered, such as the resort to a small TSK GSWP guard column for the rapid separation of affinity-purified [3H]TA-receptor complexes from free eluting steroid, and to a preparative TSK G4000SW column for the fractionation of significant amounts of the two untransformed receptor forms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
403
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Improved Stokes radius measurement of the glucocorticoid receptor using TSK G4000SW and TSK G3000SW high-performance size-exclusion columns. Analytical and preparative applications.
pubmed:affiliation
Laboratoire de Biochimie Structurale, Faculté de Médecine, Lille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't