Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1987-12-30
|
| pubmed:abstractText |
Spleen cells of Biozzi HL mouse (selection V) immunized with bovine albumin-triiodothyronine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells. Thirteen monoclonal antibodies, selected by an enzyme-linked immunosorbent assay and a radioimmunoassay, were produced in mouse ascites fluid, purified and analyzed. All of them were IgG1 (kappa). The cross-reactivity of all these monoclonal antibodies with thyroxine (T4) was less than 0.2%. The association constant determined by Scatchard analysis ranged from 1.5 x 10(9) M-1 to 2.7 x 10(10) M-1.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0272-457X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
97-101
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3679256-Animals,
pubmed-meshheading:3679256-Antibodies, Monoclonal,
pubmed-meshheading:3679256-Antigen-Antibody Complex,
pubmed-meshheading:3679256-Cell Line,
pubmed-meshheading:3679256-Cross Reactions,
pubmed-meshheading:3679256-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3679256-Hybridomas,
pubmed-meshheading:3679256-Mice,
pubmed-meshheading:3679256-Plasmacytoma,
pubmed-meshheading:3679256-Radioimmunoassay,
pubmed-meshheading:3679256-Triiodothyronine
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Production and partial characterization of monoclonal antibodies against 3,3',5-triiodo-L-thyronine.
|
| pubmed:affiliation |
Centre de Recherches Clin-Midy, Groupe Sanofi, Montpellier, France.
|
| pubmed:publicationType |
Journal Article
|