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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1987-12-7
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pubmed:databankReference |
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pubmed:abstractText |
A long (147 base pairs), natural A.T rich polypyrimidine/polypurine tract has been found 55 base pairs downstream of a chicken embryonic myosin heavy chain (MHC) gene. Analysis at the nucleotide level of nicks induced by S1 and Neurospora crassa nucleases indicate that this long interrupted polypyrimidine/polypurine tract exists in an alternate DNA structure in vitro at pH 4.5 and pH 7.5 in both supercoiled and linear plasmid DNA. The polypyrimidine/polypurine tract induces this alternate structure upon at least 200 base pairs of its 5' flanking DNA, and thus extends into the 3' coding and non-coding regions of the neighboring MHC gene. The different nicking patterns induced by the nucleases S1 and N. crassa on each strand of this alternate structure suggests that the polypyrimidine/polypurine tract may form heteronomous DNA. When this long polypyrimidine/polypurine tract is present in a supercoiled plasmid at low pH, a new and as yet undefined S1 hypersensitive DNA alteration was detected near the center of this tract.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3671071-16453673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3671071-2411549,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3671071-7196501
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8069-85
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:3671071-Animals,
pubmed-meshheading:3671071-Base Sequence,
pubmed-meshheading:3671071-Chick Embryo,
pubmed-meshheading:3671071-DNA, Recombinant,
pubmed-meshheading:3671071-DNA, Superhelical,
pubmed-meshheading:3671071-Genes,
pubmed-meshheading:3671071-Molecular Sequence Data,
pubmed-meshheading:3671071-Myosins,
pubmed-meshheading:3671071-Nucleic Acid Conformation
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pubmed:year |
1987
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pubmed:articleTitle |
A long polypyrimidine/polypurine tract induces an altered DNA conformation on the 3' coding region of the adjacent myosin heavy chain gene.
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pubmed:affiliation |
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.
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pubmed:publicationType |
Journal Article
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