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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1987-11-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have cloned cDNA of a testis-specific histone, TH2B (a variant of H2B), and rat somatic H2B gene to investigate regulation of testis-specific histone genes during rat spermatogenesis. The amino acid sequences deduced from DNA sequences show extensive sequence divergence in the N-terminal third of the two histones. The rest is highly conserved. One cysteine residue was found in TH2B. No cysteine is present in somatic histones except in H3 histone. We investigated the expression of TH2B and H2B genes using the regions of sequence divergence as hybridization probes. The TH2B gene is expressed only in the testis, and the expression of this gene is detected 14 days after birth, reaching a maximum at Day 20. The level of H2B mRNA shows a reciprocal pattern. This contrasting pattern can be explained by the gradually changing proportion of spermatogonia and spermatocytes with testicular maturation. In situ cytohybridization studies show that H2B gene is expressed primarily in proliferating spermatogonia and preleptotene spermatocytes, whereas TH2B gene is expressed exclusively in pachytene spermatocytes which first appear in testis about 14 days after birth. H2B and TH2B genes appear to be ideal markers for the study of proliferation and differentiation events in spermatogenesis and their regulatory mechanisms.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0012-1606
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
124
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
23-34
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3666307-Amino Acid Sequence,
pubmed-meshheading:3666307-Animals,
pubmed-meshheading:3666307-Base Sequence,
pubmed-meshheading:3666307-Cloning, Molecular,
pubmed-meshheading:3666307-DNA,
pubmed-meshheading:3666307-DNA, Recombinant,
pubmed-meshheading:3666307-Gene Expression Regulation,
pubmed-meshheading:3666307-Histones,
pubmed-meshheading:3666307-Male,
pubmed-meshheading:3666307-Molecular Sequence Data,
pubmed-meshheading:3666307-Nucleic Acid Hybridization,
pubmed-meshheading:3666307-RNA, Messenger,
pubmed-meshheading:3666307-Rats,
pubmed-meshheading:3666307-Rats, Inbred Strains,
pubmed-meshheading:3666307-Sertoli Cells,
pubmed-meshheading:3666307-Spermatogenesis,
pubmed-meshheading:3666307-Spermatozoa,
pubmed-meshheading:3666307-Testis,
pubmed-meshheading:3666307-Transcription, Genetic
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Molecular cloning and differential expression of somatic and testis-specific H2B histone genes during rat spermatogenesis.
|
| pubmed:affiliation |
Department of Biochemistry, University of North Carolina, Chapel Hill 27514.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|