Linked Life Data

3665842

Source:http://linkedlifedata.com/resource/pubmed/id/3665842

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-12-7
pubmed:abstractText
Glucocorticoids regulate responsiveness of many different cells and tissues to hormones that act by stimulating adenylyl cyclase. In a rat pituitary cell line, GH3, exposure to dexamethasone (150 mM for 72 h) doubled the specific activity of adenylyl cyclase in membrane extracts, whether the enzyme was stimulated by guanine nucleotides, vasoactive intestinal peptide, or forskolin. Dexamethasone treatment of GH3 cells also caused a 5-fold increase in RNA encoding the alpha-chain of Gs (alpha s) as well as a 2-fold increase in membrane-bound alpha s-polypeptide, measured by either of two assays: 1) Biochemical complementation of the alpha s deficiency of S49 lymphoma cyc- membranes in vitro; 2) Cholera toxin-catalyzed ADP ribosylation of 45- and 52-kilodalton alpha s-polypeptides. These observations suggest that glucocorticoid-induced enhancement of adenylyl cyclase activity in GH3 cells is mediated, at least in part, by increased expression of alpha s.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1711-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Dexamethasone increases adenylyl cyclase activity and expression of the alpha-subunit of Gs in GH3 cells.
pubmed:affiliation
Department of Pharmacology, University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't