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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1987-11-18
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pubmed:abstractText |
A new improved method for purification of the enzyme 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase (EC 2.3.1.67) from rat spleen is described. The catalytic subunit of cyclic AMP-dependent protein kinase in the presence of MgATP stimulated about 3-fold the activity of this partially purified enzyme activity. When [gamma-32P]ATP was included in the assay mixture, the analysis of phosphoprotein products by SDS/polyacrylamide-gel electrophoresis and autoradiography showed the incorporation of [32P]phosphate into a single protein band of about 30 kDa. Analysis of the phosphorylated amino acids indicated that the phosphate was incorporated into a serine residue. Activation of the acetylation reaction by the protein kinase was reversible. The reversal of the activation was coincident with the loss of the [32P]phosphate incorporated into the 30 kDa protein band, which suggests that the acetyltransferase is regulated by a phosphorylation-dephosphorylation mechanism dependent on cyclic AMP.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-191877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-192223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3007498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3106340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3800896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3800912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3873963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3924120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-3932094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-4061830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-6146309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-6246487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-6261830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-6732789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-6946510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-7055592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-7061484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-7430122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663199-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
245
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
893-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3663199-Acetyltransferases,
pubmed-meshheading:3663199-Amino Acids,
pubmed-meshheading:3663199-Animals,
pubmed-meshheading:3663199-Chromatography, Gel,
pubmed-meshheading:3663199-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3663199-Enzyme Activation,
pubmed-meshheading:3663199-Phosphorylation,
pubmed-meshheading:3663199-Protein Kinases,
pubmed-meshheading:3663199-Rats,
pubmed-meshheading:3663199-Spleen
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pubmed:year |
1987
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pubmed:articleTitle |
Phosphorylation of partially purified 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine:acetyl-CoA acetyltransferase from rat spleen.
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pubmed:affiliation |
Laboratorio de Nefrologia, Instituto de Investigaciones Medicas de la Fundacion Jimenez Diaz, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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