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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1987-11-6
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pubmed:abstractText |
A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a beta-band at 530 nm. In the reduced state the Bengal-gram haemoprotein showed reactivity towards CO, nitrite and hydroxylamine. SDS/polyacrylamide-slab-gel electrophoresis showed that the haemoprotein has Mr 78,000. Gel-filtration and ultracentrifugal analyses suggest that the Bengal-gram haemoprotein is oligomeric in nature. Since it differs from photosynthetic membrane cytochrome b-559 in solubility in buffer, in reactivity towards CO and in molecular size, it appears to be a novel haemoprotein b-559.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-13977264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-3915919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-4340061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-4371472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-4705134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-500668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-5160696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-5954064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-6061959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-6706983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3663098-7372715
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
243
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
723-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum).
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pubmed:affiliation |
Department of Food Chemistry, Central Food Technological Research Institute, Mysore, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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