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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1987-11-6
|
| pubmed:abstractText |
Previously we described a perfusion system for production of high yields of monoclonal antibodies in a fermentor. This system incorporated a cylindrically shaped, stainless steel filter mounted around the stirring shaft for retention of cells within a 1 liter fermentor. Modification of this filter by increasing the pore size from 5 micron to 10 micron decreased its tendency to clog and allowed continuous operation for about 3 weeks. Fresh culture medium, containing 6.5 mg glucose/ml and 3% horse serum, was supplied continually at two different perfusion rates, 850 and 1100 ml/day. Spent culture medium containing monoclonal antibody was harvested concomitantly. Highest cell density (5 X 10(7)/ml) and best antibody yield (1.7 g/l culture per day) were obtained at the higher feeding rate.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
102
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
275-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1987
|
| pubmed:articleTitle |
Effect of feeding rate on monoclonal antibody production in a modified perfusion-fed fermentor.
|
| pubmed:affiliation |
Department of Biochemistry and Microbiology, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, NJ 08903.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|