Linked Life Data

3653031

Source:http://linkedlifedata.com/resource/pubmed/id/3653031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-11-4
pubmed:abstractText
Glucose transport in rat uterus was investigated in order to determine whether the stimulation of transport by estradiol was the result of an increase in the amount of glucose transport protein in plasma membranes. In cycling rats, transport was highest during proestrus, the day on which serum estradiol concentrations are highest. In ovariectomized rats, the stimulation of transport was estrogen specific and was nearly complete within 2-3 h after injection of 0.1 microgram estradiol per animal. Estradiol stimulation resulted in a 2- to 3-fold increase in Vmax of 2-deoxy-D-glucose transport with no significant change in Michaelis-Menten constant (Km). Inhibition of the stimulation by cycloheximide or actinomycin D could not be demonstrated, although cycloheximide treatment did cause an increase in the basal rate of transport up to that observed with estradiol treatment. Upon polyacrylamide gel electrophoresis followed by Western blotting, a single protein in uterine plasma membranes reacted with a rabbit antiserum made against purified human erythrocyte glucose transport protein. This protein, believed to be the glucose transport protein, had a mol wt of 49,000 and appeared as a broad band as expected for a glycoprotein. It was found by a protein A-linked immunoassay that the amount of transport protein in uterine plasma membranes was 3.2 +/- 0.4% of the amount in human erythrocyte ghosts. Estradiol treatment had no effect on this value. These data suggest that the stimulation of glucose transport by estradiol occurred by an increase in the intrinsic activity of the transport protein rather than by an increase in the amount of transport protein in plasma membranes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1366-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Estradiol stimulation of glucose transport in rat uterus.
pubmed:affiliation
Department of Biochemistry, Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.