Linked Life Data

3652209

Source:http://linkedlifedata.com/resource/pubmed/id/3652209

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-11-16
pubmed:abstractText
The structure of a Xenopus U6 gene promoter has been investigated. Three regions in the 5'-flanking sequences of the gene that are important for U6 expression are defined. Deletion of the first, between positions -156 and -280 relative to the site of transcription initiation, reduces transcription to roughly 5% of its original level. Deletion of the second, between -60 and -77, abolishes transcription. These regions contain not only functional but also sequence homology to the previously defined distal and proximal sequence elements (DSE and PSE) of the Xenopus U2 promoter, although U2 is transcribed by RNA polymerase II and U6 by RNA polymerase III. Competition experiments show that at least the distal sequence elements of the two promoters bind to a common factor both in vivo and in vitro. Part of the sequence recognized by this factor is the octamer motif (ATG-CAAAT). A sequence similar to the common RNA polymerase II TATA box is also shown to have an effect, albeit minor, on U6 transcription. The U6 coding region contains a good match to the A box, part of all previously characterized RNA polymerase III promoters. Deletion of this region has no apparent effect on the efficiency or accuracy of U6 transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:3652209-Animals, pubmed-meshheading:3652209-Base Sequence, pubmed-meshheading:3652209-Binding, Competitive, pubmed-meshheading:3652209-DNA-Directed RNA Polymerases, pubmed-meshheading:3652209-Female, pubmed-meshheading:3652209-Humans, pubmed-meshheading:3652209-Mutation, pubmed-meshheading:3652209-Oligodeoxyribonucleotides, pubmed-meshheading:3652209-Promoter Regions, Genetic, pubmed-meshheading:3652209-RNA, Small Nuclear, pubmed-meshheading:3652209-RNA Polymerase II, pubmed-meshheading:3652209-RNA Polymerase III, pubmed-meshheading:3652209-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:3652209-Sequence Homology, Nucleic Acid, pubmed-meshheading:3652209-Transcription, Genetic, pubmed-meshheading:3652209-Transcription Factors, pubmed-meshheading:3652209-Xenopus laevis
pubmed:year
1987
pubmed:articleTitle
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
pubmed:affiliation
Institut de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France.
pubmed:publicationType
Journal Article, Comparative Study