Linked Life Data

3651556

Source:http://linkedlifedata.com/resource/pubmed/id/3651556

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-11-18
pubmed:abstractText
Total internal reflection of exciting light, in combination with fluorescence intensity and polarization measurements, was used to selectively study fluorescent compounds adsorbed to the interface region between two immiscible liquids. A fluorometer was constructed which provided excitation at variable angles of incidence and allowed sensitive detection of polarized fluorescence emitted from the interface. The compound 4,4'-bis-1-phenylamino-8-naphthalenesulfonate (bis-ANS) was examined at a decalin/water interface and was found to possess remarkable affinity for the interface region with the bulk of the adsorbed molecule residing in the decalin phase. The adsorbed fluorophore displayed an apparent hindered rotation in the plane of the interface with a rotational diffusion coefficient 3- to 12-fold lower than that expected for bis-ANS in solution. While other dyes examined were not found to be significantly surface active, the addition of cationic surfactant sufficed to induce adsorption of the anionic fluorophore 1-aminonaphthalene-3,6,8-trisulfonic acid. This fluoropore was found to reside in an aqueous environment when bound to the interface, and it also exhibited hindered rotation in the plane of the interface. As the concentrations of the dyes were increased, both adsorbed dyes exhibited polarization reductions consistent with excitation energy transfer. Adsorption of bis-ANS was reversed by addition of bovine serum albumin. The membrane protein cytochrome b5 was found not to bind at the decalin/water interface, indicating that interaction with lipid is required for its adherence to biological membranes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-1102611, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-273241, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-4404746, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-5388144, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-6378070, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-6518253, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-6518254, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-6804467, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-6838837, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-7014571, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-70452, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-7082639, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-7194696, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-73542, http://linkedlifedata.com/resource/pubmed/commentcorrection/3651556-7356973
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
367-79
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Biological membrane modeling with a liquid/liquid interface. Probing mobility and environment with total internal reflection excited fluorescence.
pubmed:affiliation
Department of Biochemistry, School of Chemical Sciences, University of Illinois, Urbana 61801.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.