| pubmed-article:3632693 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C0018956 | lld:lifeskim |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C1514185 | lld:lifeskim |
| pubmed-article:3632693 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:3632693 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3632693 | pubmed:dateCreated | 1987-10-19 | lld:pubmed |
| pubmed-article:3632693 | pubmed:abstractText | A method to purify pluripotent hemopoietic stem cells in the Go phase from mouse bone marrow was established. Bone marrow cells from 5-fluorouracil (5-FU)-treated mice were fractionated by Percoll density gradient. The cells with density between 1.063 and 1.075 were further separated into wheat germ agglutinin (WGA)-positive and -negative cells using fluorescent-activated cell sorter (FACS) after staining with fluorescein isothiocyanate-conjugated WGA (FITC-WGA). An assay for spleen colony-forming units (CFU-S) revealed that the WGA-positive cells (1 X 10(6)) produced 1380 CFU-S (about 150 times of the number in the original bone marrow cells) on day 12 (but no CFU-S on day 8), whereas the WGA-negative cells produced no CFU-S. Thus, the stem cells in the Go phase are found to be enriched 150 times in 5-FU-treated WGA-positive cells. | lld:pubmed |
| pubmed-article:3632693 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3632693 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3632693 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3632693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3632693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3632693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3632693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3632693 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3632693 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3632693 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:OgataHH | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:TojoSS | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:SugiuraKK | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:KulpCC | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:IkeharaSS | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:YasumizuRR | lld:pubmed |
| pubmed-article:3632693 | pubmed:author | pubmed-author:Miyama-InabaM... | lld:pubmed |
| pubmed-article:3632693 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3632693 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3632693 | pubmed:volume | 147 | lld:pubmed |
| pubmed-article:3632693 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3632693 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3632693 | pubmed:pagination | 687-94 | lld:pubmed |
| pubmed-article:3632693 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:3632693 | pubmed:meshHeading | pubmed-meshheading:3632693-... | lld:pubmed |
| pubmed-article:3632693 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3632693 | pubmed:articleTitle | Isolation of murine pluripotent hemopoietic stem cells in the Go phase. | lld:pubmed |
| pubmed-article:3632693 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3632693 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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