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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-10-19
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pubmed:abstractText |
A method to purify pluripotent hemopoietic stem cells in the Go phase from mouse bone marrow was established. Bone marrow cells from 5-fluorouracil (5-FU)-treated mice were fractionated by Percoll density gradient. The cells with density between 1.063 and 1.075 were further separated into wheat germ agglutinin (WGA)-positive and -negative cells using fluorescent-activated cell sorter (FACS) after staining with fluorescein isothiocyanate-conjugated WGA (FITC-WGA). An assay for spleen colony-forming units (CFU-S) revealed that the WGA-positive cells (1 X 10(6)) produced 1380 CFU-S (about 150 times of the number in the original bone marrow cells) on day 12 (but no CFU-S on day 8), whereas the WGA-negative cells produced no CFU-S. Thus, the stem cells in the Go phase are found to be enriched 150 times in 5-FU-treated WGA-positive cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
147
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
687-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3632693-Animals,
pubmed-meshheading:3632693-Bone Marrow,
pubmed-meshheading:3632693-Bone Marrow Cells,
pubmed-meshheading:3632693-Cell Separation,
pubmed-meshheading:3632693-Centrifugation, Density Gradient,
pubmed-meshheading:3632693-Colony-Forming Units Assay,
pubmed-meshheading:3632693-Female,
pubmed-meshheading:3632693-Flow Cytometry,
pubmed-meshheading:3632693-Fluorescein-5-isothiocyanate,
pubmed-meshheading:3632693-Fluoresceins,
pubmed-meshheading:3632693-Fluorouracil,
pubmed-meshheading:3632693-Hematopoietic Stem Cells,
pubmed-meshheading:3632693-Interphase,
pubmed-meshheading:3632693-Mice,
pubmed-meshheading:3632693-Mice, Inbred C3H,
pubmed-meshheading:3632693-Microscopy, Electron,
pubmed-meshheading:3632693-Wheat Germ Agglutinins
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation of murine pluripotent hemopoietic stem cells in the Go phase.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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