Linked Life Data

3632681

Source:http://linkedlifedata.com/resource/pubmed/id/3632681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-10-8
pubmed:abstractText
Infrared spectroscopy of highly purified fractions of plasma membrane vesicles from hypocotyls of etiolated soybean (Glycine max L.) seedlings revealed changes in bands assigned to proteins and phospholipids upon exposure to the growth hormone analog, 2,4-dichlorophenoxyacetic acid (2,4-D). The changes included a concentration dependent broadening of amide I absorbance and a change in the absorbance ratios of amide I and amide II indicative of a change in protein conformation. Band broadening of amide I was observed at 2,4-D concentrations as low as 10(-8) M, and the optimal 2,4-D concentration to evoke the change was 1 microM whereas the amide peak ratios (amide II/amide I) declined steadily over the range of concentrations (10(-8) to 10(-3)M) tested. An alteration in hydrocarbon chains (CH2 scissoring) was seen only at 1 mM (10(-3) M) 2,4-D. In contrast, the vibrational frequency of the choline stretch declined proportionally over the range 10(-6) to 10(-3). The findings provide evidence for a conformational change in the plasma membrane in response to the hormone demonstrable in a cell-free system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
506-12
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Infrared spectroscopic evidence for a conformational alteration of plant plasma membranes upon exposure to the growth hormone analog, 2,4-dichlorophenoxyacetic acid.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.