Linked Life Data

3626615

Source:http://linkedlifedata.com/resource/pubmed/id/3626615

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-10-15
pubmed:abstractText
A method is described for dispersal and subsequent maintenance in acute culture of neurones dissociated from ganglia of the adult cockroach (Periplaneta americana) and brain of the adult fly (Musca domestica). The effects of different media on the survival and attachment to different substrates have been investigated. About 25% of dissociated neurones survived well for periods greater than 6 days in a cockroach saline supplemented with foetal calf serum and the antibiotics fungizone and gentamicin. Fly neurones were more difficult to disperse. Fly saline supplemented with foetal calf serum and the antibiotics fungizone and gentamicin proved a successful short-term culture medium. Survival times were shorter, most neurones dying after 5 days. The demonstrated viability of such neurones has considerable potential for insecticide mode of action studies using the patch-clamp technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-202
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Dissociation and maintenance in vitro of neurones from adult cockroach (Periplaneta americana) and housefly (Musca domestica).
pubmed:publicationType
Journal Article