Linked Life Data

3621597

Source:http://linkedlifedata.com/resource/pubmed/id/3621597

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1987-10-22
pubmed:abstractText
Existing methods for the estimation of the branched-chain amino acids (BCAA) normally require sophisticated, expensive instrumentation. An alternative is an enzymatic spectrophotometric method but this requires relatively large volumes of blood and is rather time-consuming. A simple enzymatic fluorimetric method for the measurement of the BCAA in microliter samples of plasma is described here. The method is based on the oxidative deamination of L-leucine, L-isoleucine and L-valine by leucine dehydrogenase from Bacillus species. The NADH which is formed in stoichiometric quantities is estimated fluorimetrically. In the presence of the ketone-trapping agent hydrazine the reaction goes to completion in an alkaline incubation medium at 37 degrees C. By this method the combined BCAA can be measured routinely in 20 microliter sample of plasma. The test exhibits acceptable precision and reproducibility.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A simple enzymatic fluorimetric method for the determination of branched-chain L-amino acids in microlitre volumes of plasma.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't