|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1987-10-16
|
|
pubmed:abstractText |
Eight samples of human liver have been characterised for microsomal protein content, cytochrome P-450 content, tolbutamide 4-hydroxylase and ethinyloestradiol 2-hydroxylase activities. Cytochrome P-450 content correlated significantly with ethinyloestradiol 2-hydroxylase activity but not with tolbutamide 4-hydroxylase activity. There was no significant correlation between ethinyloestradiol 2-hydroxylase and tolbutamide 4-hydroxylase activities. The maximum tolbutamide 4-hydroxylase activity was 0.45 nmol min-1 mg-1 microsomal protein, with a Km value of 74 microM. A number of compounds were tested for their ability to inhibit tolbutamide metabolism. All the compounds showing inhibition were either non-competitive or mixed non-competitive inhibitors of tolbutamide 4-hydroxylation. These studies suggest that tolbutamide is metabolised by an isozyme of cytochrome P-450 which appears to be distinct from those isozymes metabolising many other drugs.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-14071924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-14209971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-3555579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-3724146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-3739363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-401727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-4027115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-448864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-5014495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-5968015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-6121708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-6147254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-6488689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-6626444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-6697063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-7042170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-7213514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3620298-913025
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0306-5251
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
230-4
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3620298-Adolescent,
pubmed-meshheading:3620298-Adult,
pubmed-meshheading:3620298-Aged,
pubmed-meshheading:3620298-Child,
pubmed-meshheading:3620298-Child, Preschool,
pubmed-meshheading:3620298-Cytochrome P-450 CYP1A1,
pubmed-meshheading:3620298-Cytochrome P-450 Enzyme System,
pubmed-meshheading:3620298-Female,
pubmed-meshheading:3620298-Humans,
pubmed-meshheading:3620298-Isoenzymes,
pubmed-meshheading:3620298-Kinetics,
pubmed-meshheading:3620298-Male,
pubmed-meshheading:3620298-Microsomes, Liver,
pubmed-meshheading:3620298-Middle Aged,
pubmed-meshheading:3620298-Mixed Function Oxygenases,
pubmed-meshheading:3620298-Proteins,
pubmed-meshheading:3620298-Steroid Hydroxylases,
pubmed-meshheading:3620298-Tolbutamide
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Tolbutamide 4-hydroxylase activity of human liver microsomes: effect of inhibitors.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
