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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6131
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pubmed:dateCreated |
1987-9-18
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pubmed:abstractText |
Human platelet-derived growth factor (PDGF) is a potent mitogenic polypeptide which is believed to be a heterodimer of A- and B-chains stabilized by interchain disulphide bonds. The B-chain of PDGF is encoded by the c-sis gene, the normal cellular homologue of the transforming gene of the simian sarcoma virus (SSV). cDNA clones of the B-chain from both normal and transformed cells have mutually consistent DNA sequences. Recently, an A-chain cDNA clone (D-1) was isolated from a transformed human glial cell cDNA library. We report the complete sequence of an A-chain cDNA clone (BT-1) isolated from a normal human umbilical vein endothelial (HUVE) cell cDNA library. BT-1 differs from the sequence of the D-1 clone by a 69 base pair deletion containing the predicted carboxy terminus of the protein. The mRNA levels of the A- and B-chains of PDGF in HUVE cells were analysed and shown to respond differently to the endothelial cell growth factor (ECGF).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:issn |
0028-0836
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
328
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
619-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3614363-Animals,
pubmed-meshheading:3614363-Base Sequence,
pubmed-meshheading:3614363-Cell Line,
pubmed-meshheading:3614363-DNA,
pubmed-meshheading:3614363-Endothelium,
pubmed-meshheading:3614363-Fibroblast Growth Factors,
pubmed-meshheading:3614363-Humans,
pubmed-meshheading:3614363-Macromolecular Substances,
pubmed-meshheading:3614363-Neuroglia,
pubmed-meshheading:3614363-Nucleic Acid Hybridization,
pubmed-meshheading:3614363-Platelet-Derived Growth Factor,
pubmed-meshheading:3614363-RNA, Messenger,
pubmed-meshheading:3614363-Umbilical Veins
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pubmed:articleTitle |
cDNA clones reveal differences between human glial and endothelial cell platelet-derived growth factor A-chains.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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