Linked Life Data

3611

Source:http://linkedlifedata.com/resource/pubmed/id/3611

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-5-18
pubmed:abstractText
The sensitivity and the specificity of the Farr assay for the detection of antibodies to double stranded (ds) DNA depends very much on the reaction conditions. The interaction between ds DNA and anti-ds DNA is inhibited when ionic strength and pH are increased. ds DNA is bound by normal sera at ionic strength lower than 0.11 M NaCl and at physiological ionic strength when the pH is lower than 7.2. Substantial binding of DNA by normal serum takes place in barbitone, borate or Tris-HCl buffers at concentrations of 30 mM or higher, even at a pH higher than 7.2. Such binding is due to Clq and is only partially prevented by heating the serum for 30 min at 56 degrees C, but 10 mM phosphate in the incubation mixture completely prevents it. Standardization of ionic strength, pH, phosphate concentration, incubation volume and DNA-serum ratio enhances the diagnostic usefulness of the Farr assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-37
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Immunology of DNA. I. The influence of reaction conditions on the Farr assay as used for the detection of anti-ds DNA.
pubmed:publicationType
Journal Article