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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-7-25
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pubmed:abstractText |
The hepatic microsomal haem oxygenase activity of rats treated with CoCl2 was studied kinetically by measuring biliverdin, the immediate product of the reaction. Biliverdin was extracted with diethyl ether/ethanol mixture, and was determined by the difference between A690 and A800. The apparent Km value for NADPH (at 50 microM-haematin) was about 0.2 microM when an NADPH-generating system was used, whereas that for NADH was about 630 microM. Essentially the same Vmax. values were obtained for both the NADH- and NADPH-dependent haem oxygenase reactions. No synergism was observed with NADH and NADPH. The NADH-dependent reaction was competitively inhibited by NADP+, with a Ki of about 10 microM. The inhibitoin of the NADH-dependent reaction by the antibody against rat liver microsomal NADPH-cytochrome c reductase was essentially complete, with a pattern similar to that of the NADPH-dependent reaction. The immunochemical experiment and the comparison of the kinetic values with the reported data on isolated NADH-cytochrome b5 reductase and NADPH--cytochrome c reductase indicated the involvement of the latter enzyme in NADH-dependent haem oxygenation by microsomal fraction in situ.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-13249955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-13475360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-18477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-203576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-4113125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-4386763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-4390687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-4468818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-4530983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-5158905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-5543939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-836794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-845137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/36076-96108
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
323-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:36076-Animals,
pubmed-meshheading:36076-Biliverdine,
pubmed-meshheading:36076-Cobalt,
pubmed-meshheading:36076-Electron Transport,
pubmed-meshheading:36076-Enzyme Induction,
pubmed-meshheading:36076-Heme,
pubmed-meshheading:36076-Kinetics,
pubmed-meshheading:36076-Male,
pubmed-meshheading:36076-Microsomes, Liver,
pubmed-meshheading:36076-Mixed Function Oxygenases,
pubmed-meshheading:36076-NAD,
pubmed-meshheading:36076-NADP,
pubmed-meshheading:36076-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:36076-Rats
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pubmed:year |
1979
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pubmed:articleTitle |
Electron-transport pathway of the NADH-dependent haem oxygenase system of rat liver microsomal fraction induced by cobalt chloride.
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pubmed:publicationType |
Journal Article,
In Vitro
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