Linked Life Data

3607000

Source:http://linkedlifedata.com/resource/pubmed/id/3607000

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1987-8-31
pubmed:abstractText
The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size-exclusion high-performance liquid chromatography and sedimentation equilibrium studies indicated that acetylated inner core histones associate to species larger than the octamer and form a more stable complex. Circular dichroism studies demonstrated that the amount of alpha-helix increases with increasing association of the histones. Furthermore, acetylation results in an increase in the amount of alpha-helix, perhaps coupled through its effect on the association state. At high protein concentration and elevated temperature, the acetylated sample displays a greater increase in beta-sheet content, relative to the control sample. This increase in beta-sheet content may be induced during the association of the acetylated sample to species larger than the octamer. There is a marked effect on the conformation of both acetylated and control inner core histones as a function of protein concentration, ionic strength, and temperature. The difference in conformational flexibility and association state of the acetylated vs. the control inner histone core may play a significant role in the control of transcription in the nucleus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2944-55
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Structural studies of acetylated and control inner core histones.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't