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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1987-8-12
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pubmed:abstractText |
Three methods have been developed for measuring pseudo-alpha- and pseudo-beta-DL-glucose (pseudo-beta-D-glucose), synthetic compounds in which the ring oxygens of alpha- and beta-DL-glucose (beta-D-glucose) have been replaced by a methylene group. Moderate sensitivity in the determination of these pseudo-glucoses dissolved in human serum was obtained by GLC (0.1 nmol) and HPLC (0.5 nmol). The colorimetric determination with glucose 2-oxidase, peroxidase, and 2,2'-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) was satisfactory for the assay of pseudo-alpha- and pseudo-beta-DL-glucose (respective sensitivities: 25 and 5 nmol). The addition of hexokinase to the colorimetric assay system made it possible to eliminate glucose present in the sample, such as serum, and the remaining pseudo-alpha- or pseudo-beta-DL-glucose in the sample solution could then be measured by a colorimetric method using glucose 2-oxidase. The methods described can be used for biochemical studies involving pseudo-alpha- and pseudo-beta-DL-glucose.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
378-83
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3605605-Chromatography, Gas,
pubmed-meshheading:3605605-Chromatography, High Pressure Liquid,
pubmed-meshheading:3605605-Colorimetry,
pubmed-meshheading:3605605-Glucose,
pubmed-meshheading:3605605-Glucose Oxidase,
pubmed-meshheading:3605605-Humans,
pubmed-meshheading:3605605-Stereoisomerism
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pubmed:year |
1987
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pubmed:articleTitle |
Determination of pseudo-alpha- and pseudo-beta-DL-glucose by gas-liquid chromatography, high-performance liquid chromatography, and enzymatic colorimetry with glucose 2-oxidase.
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pubmed:publicationType |
Journal Article
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