Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-8-10
pubmed:abstractText
Poly-L-ornithine has been used to introduce DNA and RNA into mammalian cells in culture. Ornithine-mediated DNA transfer has several interesting and potentially useful properties. The procedure is technically straightforward and is easily applied to either small or large numbers of recipient cells. The efficiency of transformation is high. Under optimal conditions, 1 to 2% of recipient mouse L cells take up and continue to express selectable marker genes. DNA content of transformants can be varied reproducibly, yielding cells with just one or two copies of the new gene under one set of conditions, while under a different set of conditions 25 to 50 copies are acquired. Cotransformation and expression of physically unlinked genes occur at high efficiency under conditions favoring multiple-copy transfer. Polyornithine promotes gene transfer into cell lines other than L cells. These include Friend erythroleukemia cells and NIH 3T3 cells. Both are transformed about 1 order of magnitude more efficiently by this procedure than by standard calcium phosphate products. However, the method does not abolish the large transformation efficiency differences between these cell lines that have been observed previously by other techniques. (vi) mRNA synthesized in vitro was also introduced into cells by this method. The RNA was translated resulting in a transient accumulation of the protein product.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-13989646, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-14056989, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-168973, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-184963, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-187721, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-194704, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-208776, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-210957, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-279411, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-3466022, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-3466023, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-3860826, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-386338, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-4297402, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-4299537, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-4320570, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-493123, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-568565, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-601681, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6089359, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6207484, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6244898, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6246525, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6254080, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6256082, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6278479, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6296830, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6298741, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6316255, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6358861, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6438633, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-6931407, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-7414320, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-7460013, http://linkedlifedata.com/resource/pubmed/commentcorrection/3600662-912752
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2286-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Poly-L-ornithine-mediated transformation of mammalian cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't