Linked Life Data

3596992

Source:http://linkedlifedata.com/resource/pubmed/id/3596992

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1987-8-4
pubmed:abstractText
Twenty-five human eyes of various ages from eye bank donors and surgical enucleations were obtained for ultrastructural cytochemical demonstration of acid phosphatase (AcPase) and arylsulfatase B (ASB) in the retinal pigment epithelium (RPE) and Bruch's membrane. Results with post-mortem (less than 10 hr) tissues were comparable to those of fresh specimens. Vigorous reactivity was demonstrated in lysosomes of RPE and choriocapillary endothelium but no reactive sites were found in Bruch's membrane, although many lysosome-like dense bodies occurred in eyes greater than 20 yr of age. Granular drusen of 30-70-yr-olds contained no reactive bodies. In eyes greater than 80 years old blebs of RPE basal cytoplasm protruding into Bruch's membrane contained reactive lysosomes. We conclude that the RPE ordinarily does not extrude or exocytose active lysosomes (ie, phagolysosomes, other secondary lysosomes, residual bodies, lipofuscin) or lysosomal enzymes. Aged RPE, however, extrudes cytoplasm with active lysosomes into Bruch's membrane. The possible impact of this process on the extracellular connective tissue is discussed, particularly with regard to age-related deterioration of Bruch's membrane and neovascularization.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1138-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Lysosomal enzyme cytochemistry of human RPE, Bruch's membrane and drusen.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't