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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1987-7-29
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pubmed:abstractText |
We describe a simplified procedure for purification of Clostridium difficile toxin B. In this procedure, cytotoxicity is associated with a single protein band with a molecular mass of 230 kilodaltons. We used direct fluorescent staining of actin filaments to study the effect of this toxin on cultured cells. Morphologic changes were preceded by a decrease in the number and length of stress fibers followed by their disappearance with condensation of cellular actin around the nucleus. We then showed that cells treated with either cytochalasin B or toxin B had a significant increase in the monomeric actin pool as quantitated by DNase I inhibition. In contrast to the cytochalasins, toxin B had no direct effect on the rate or extent of actin polymerization or network formation in vitro. Cytoplasmic extracts of toxin B-treated cells had a significantly lower level of modulating activity on actin assembly and interactions in vitro compared with extracts of untreated cells. These results suggest that the action of toxin B on cells is due to direct or indirect effects on cellular proteins involved in controlling the state of actin assembly in the cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-287078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-291981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-3022284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-3030380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-3940653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-3944088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-4254541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6172384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6628564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6802855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6809404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6892818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6892916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-6893016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-700321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-7010532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-7085078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-728995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-7333662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-7356663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3596804-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1610-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
Biochemical studies on the effect of Clostridium difficile toxin B on actin in vivo and in vitro.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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