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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1987-8-20
|
| pubmed:abstractText |
Monoclonal autoantibodies obtained using the native porcine thyroglobulin (Tg) molecule for immunization were shown to react against a defined epitope of the Tg molecule. Its biochemical characterization was undertaken, using trypsin treatment of Tg, followed by gel filtration, affinity chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high pressure liquid chromatography analysis. Immunoreactivity tested by immunoblotting with appropriate monoclonal anti-Tg antibody allowed the detection of one peptide fragment of 5-10 kDa, after limited proteolysis with trypsin. We demonstrated that this peptide reacts exclusively with monoclonal anti-Tg antibodies which block in vitro proliferative T cell response; moreover, the 5-10-kDa tryptic fragments from porcine Tg were able to induce thyroiditis when injected into normal CBA mice, while porcine Tg depleted from these fragments was not able to do so.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
843-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3595709-Animals,
pubmed-meshheading:3595709-Antibodies, Monoclonal,
pubmed-meshheading:3595709-Chromatography, Affinity,
pubmed-meshheading:3595709-Chromatography, Gel,
pubmed-meshheading:3595709-Chromatography, High Pressure Liquid,
pubmed-meshheading:3595709-Disease Models, Animal,
pubmed-meshheading:3595709-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3595709-Histocytochemistry,
pubmed-meshheading:3595709-Immunization,
pubmed-meshheading:3595709-Lymphocyte Activation,
pubmed-meshheading:3595709-Mice,
pubmed-meshheading:3595709-Mice, Inbred CBA,
pubmed-meshheading:3595709-Mice, Inbred Strains,
pubmed-meshheading:3595709-Molecular Weight,
pubmed-meshheading:3595709-Peptide Fragments,
pubmed-meshheading:3595709-Swine,
pubmed-meshheading:3595709-Thyroglobulin,
pubmed-meshheading:3595709-Thyroid Gland,
pubmed-meshheading:3595709-Thyroiditis, Autoimmune,
pubmed-meshheading:3595709-Trypsin
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Experimental autoimmune thyroiditis induced by a 5-10-kDa tryptic fragment from porcine thyroglobulin.
|
| pubmed:publicationType |
Journal Article
|