Linked Life Data

3594572

Source:http://linkedlifedata.com/resource/pubmed/id/3594572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-8-20
pubmed:abstractText
The growth hormone (GH) gene is specifically expressed in the anterior pituitary. The first 289 bp of the human (h)GH 5' region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH TATA box. Using in vitro transcription systems we demonstrate greater hGH promoter activity in extracts of GH-expressing GC cells than in extracts of nonexpressing HeLa cells. The two GHF-1 binding sites are essential promoter elements in GC extracts. Addition of GHF-1-containing fractions to HeLa extracts stimulates hGH promoter activity. Thus, GHF-1 appears to be a positively acting, cell-type- and promoter-specific transcription factor. Furthermore, the tissue specificity of the hGH promoter appears to be positively controlled by the abundance or activity of GHF-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
267-75
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't