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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1987-7-23
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pubmed:abstractText |
A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine and the 4-position of cytosine. These nucleotides are incorporated into DNA probes by standard nick-translation protocols. DNA probes labeled with biotin derivatives of these nucleotides are effectively hybridized to target DNA sequences and can be detected by a streptavidin and calf intestinal alkaline phosphatase conjugate with a sensitivity (0.25 pg DNA) sufficient for reproducible and rapid detection of single copy genes in a Southern blot of mammalian DNA. Also, a procedure has been developed to allow reprobing of nylon filters that have been hybridized with biotinylated probes and developed with the streptavidin/alkaline phosphatase conjugate and a standard dye system.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-1140197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-14289336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-237414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-2582358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-2865570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-2939103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-2988824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-2999191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-3714503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-5440907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-572747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-6191328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-6198634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-6204550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-6273878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-6302989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3588302-776633
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4513-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.
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pubmed:publicationType |
Journal Article
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