Linked Life Data

3586660

Source:http://linkedlifedata.com/resource/pubmed/id/3586660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-6-29
pubmed:abstractText
Dispersed cells were prepared from the anterior pituitary glands of 6-week old male and retired breeder female rats and were cultured for 3 days in a serum-containing medium which lacked estrogen. Following short-term treatments (1-24 h) with estradiol at a concentration of 10(-9) M, the levels of prolactin gene transcription were assayed by quantitating the amount of radiolabeled uridine monophosphate incorporated into prolactin mRNA sequences by pituitary cell nuclei prepared from these cultures. Estradiol significantly stimulated the transcription of the prolactin gene in these primary cultures within 6 h. Similar levels of stimulation were observed in cells from both male and female rats. Estradiol treatment had no effect on the transcription of the growth hormone gene, suggesting its stimulatory effects on prolactin gene transcription are specific. Estradiol also significantly stimulated the transcription of the prolactin gene in cultures in which protein synthesis was grossly inhibited by cycloheximide. These data illustrate that estrogen regulates the expression of the prolactin gene at the transcriptional level, through a direct action on the cells of the anterior pituitary gland, and by a mechanism which does not require the synthesis of any intermediary proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Estradiol stimulates prolactin gene transcription in primary cultures of rat anterior pituitary cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't