Linked Life Data

3585860

Source:http://linkedlifedata.com/resource/pubmed/id/3585860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
1987-6-30
pubmed:abstractText
The stability of the glucocorticoid receptor population in thymus cytosol prepared in a molybdate containing buffer was examined. Stability was initially assessed by an association time course. Those experiments demonstrated a slow decline in receptor specific binding, beginning at about eight hours of incubation at 4 degrees C. Stability was further assessed with a forward and reverse isotherm. The presence of hysteresis when the forward (association) and reverse (dissociation) isotherms were compared demonstrates that some element of the system either does not reach or does not maintain a stable state. A comparison with studies in muscle indicates that either the receptors or some component(s) of the cell free systems from thymus and muscle are different. Furthermore, equilibrium binding characteristics examined in thymus cytosols prepared in a molybdate buffer may be questionable due to instability in the receptor population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0197-5110
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
411-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Muscle and thymus cell-free glucocorticoid receptor systems are different.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S.