Linked Life Data

3582772

Source:http://linkedlifedata.com/resource/pubmed/id/3582772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1987-6-26
pubmed:abstractText
Mouse hybridomas have been produced that produce antibody that can bind to the bipyridyl herbicide paraquat with high affinity (Ka = 1.2 X 10(10) litres/mole). In addition, one cell line, 321, secretes antibody in the concentration range 60-100 micrograms/ml of culture, and 5 mg/ml in ascites fluid. Such high concentrations make it feasible to produce gram quantities of antibody with relative ease and low cost. The antibody is of class and sub-class IgG1 and can be readily purified by protein A and paraquat specific affinity chromatography. Radioimmunoassays and in vitro neutralization studies have demonstrated that 1 mg of antibody is required to bind 2.5 micrograms of paraquat. When antibody binds to paraquat in vitro it renders the paraquat unavailable for accumulation into rat lung tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
495-501
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The development of mouse monoclonal antibodies that bind and neutralize paraquat.
pubmed:publicationType
Journal Article