Linked Life Data

3582768

Source:http://linkedlifedata.com/resource/pubmed/id/3582768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1987-6-26
pubmed:abstractText
An EBV-transformed human B cell line, which produces monoclonal IgM antibodies, was cultured in an immobilized state on a ceramic matrix in the Opticell system. Cell growth, metabolic activity and product formation of these cells were optimized in a single semi-continuous culture of 10 inductions for 29 d. All in all, 19.5 g of IgM were harvested in 193 l of culture supernatant. The average production of IgM in the Opticell unit of 20 l was 0.7 g/d which was obtained even in serum-reduced medium (1%). Under optimal conditions IgM yields were enhanced to 1.5-2.0 g/d. These results indicate that the Opticell is a suitable system for the large scale production of monoclonal antibodies, particularly because the capacity for aeration and pumping of one Opticell unit is sufficient to control 3 growth chambers running in parallel.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
473-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Production of human monoclonal antibodies from immobilized cells in the Opticell culture system.
pubmed:publicationType
Journal Article