Linked Life Data

3571444

Source:http://linkedlifedata.com/resource/pubmed/id/3571444

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-5-28
pubmed:abstractText
Day 4 rabbit blastocysts were cultured in Ham's F-10 medium supplemented either with homologous serum or uterine flushings. Development was assessed by leucine and methionine incorporation at 24 h and 48 h, respectively, after initiation of culture and compared with day 4 and day 5 non-cultured controls. After 24 h in culture, incorporation data were similar for both media groups. After 48 h, a significantly higher protein synthesis activity was found in blastocysts which had access to uterine secretions in vitro. However, incorporation levels of day 5 controls were not reached. Modes of incorporation of leucine and methionine revealed to be very similar in all experimental groups but ranged on a different level. Leucine was incorporated on a 5.5-fold higher level than methionine. We conclude that (i) supplementation of culture media with uterine secretions is beneficial for blastocyst development, and (ii) further studies should focus on non-serum components within the uterine fluid and their significance for embryonic development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Quantitative aspects of protein synthesis in non-cultured and cultured rabbit blastocysts.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't