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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-6-11
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pubmed:abstractText |
The effect of K+ depletion of Hep 2 cells on ion fluxes, internal pH, cell volume, and membrane potential was studied. The cells were depleted of K+ by incubation in K+-free buffer with or without a preceding exposure to hypotonic medium. Efflux of K+ in cells not exposed to hypotonic medium was inhibited by furosemide or by incubation in Na+-free medium, indicating that in this case at least part of the K+ efflux occurs by Na+/K+/Cl- cotransport. After exposure to hypotonic medium, K+ efflux was not inhibited by furosemide, whereas it was partly inhibited by 4,4'-diisothiocyano-2,2'-stilbene-disulfonic acid (DIDS). Exposure to hypotonic medium induced acidification of the cytosol, apparently because of efflux of protons from intracellular acidic vesicles. When isotonicity was restored, a rebound alkalinization of the cytosol was induced, because of activation of the Na+/H+ antiporter. While hypotonic shock and a subsequent incubation in K+-free buffer rapidly depolarized the cells, depolarization occurred much more slowly when the K+ depletion was carried out by incubation in K+-free buffer alone. The cell volume was reduced in both cases. K+ depletion by either method strongly reduced the ability of the cells to accumulate 36Cl- by anion antiport, and K+-depleted cells were unable to increase the rate of 36Cl- uptake in response to alkalinization of the cytosol.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/4-Acetamido-4'-isothiocyanatostilben...,
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Furosemide,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Radioisotopes
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
131
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3571337-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:3571337-4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid,
pubmed-meshheading:3571337-Anions,
pubmed-meshheading:3571337-Biological Transport,
pubmed-meshheading:3571337-Carcinoma,
pubmed-meshheading:3571337-Cell Line,
pubmed-meshheading:3571337-Chlorides,
pubmed-meshheading:3571337-Cytosol,
pubmed-meshheading:3571337-Furosemide,
pubmed-meshheading:3571337-Humans,
pubmed-meshheading:3571337-Hydrogen-Ion Concentration,
pubmed-meshheading:3571337-Hypotonic Solutions,
pubmed-meshheading:3571337-Laryngeal Neoplasms,
pubmed-meshheading:3571337-Membrane Potentials,
pubmed-meshheading:3571337-Potassium,
pubmed-meshheading:3571337-Potassium Deficiency,
pubmed-meshheading:3571337-Radioisotopes
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pubmed:year |
1987
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pubmed:articleTitle |
Effect of potassium depletion of Hep 2 cells on intracellular pH and on chloride uptake by anion antiport.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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