| pubmed-article:3571289 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C0221198 | lld:lifeskim |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C0441587 | lld:lifeskim |
| pubmed-article:3571289 | lifeskim:mentions | umls-concept:C1521805 | lld:lifeskim |
| pubmed-article:3571289 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:3571289 | pubmed:dateCreated | 1987-6-19 | lld:pubmed |
| pubmed-article:3571289 | pubmed:abstractText | A gel assay is introduced to measure DNA polymerase insertion kinetics at single sites along a DNA template strand. The assay is used to analyze the kinetics of inserting deoxynucleotides opposite a synthetic abasic (apurinic/apyrimidinic) lesions using Drosophila DNA polymerase alpha. The location of the abasic lesion next to different nearest-neighbor bases allows the effects of base stacking on the specificity of insertion to be evaluated. The specificity of nucleotide insertion, Vmax/Km, is 6-11 times greater for A over G and about 20-50 times greater for A over C and T. The insertion specificity at the abasic lesion appears to depend more on differences in Vmax than Km. Apparent Michaelis constants for inserting A and G deoxynucleotides are similar to within about a factor of 2. The insertion of A or G occurs most efficiently at the abasic lesion when T is the 5'-nearest neighbor on the primer strand and least efficiently when G is the 5'-nearest neighbor. The presence of different base stacking partners adjacent to the site of insertion has up to a 4-fold effect on specificity. | lld:pubmed |
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| pubmed-article:3571289 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3571289 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3571289 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3571289 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3571289 | pubmed:month | May | lld:pubmed |
| pubmed-article:3571289 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3571289 | pubmed:author | pubmed-author:GoodmanM FMF | lld:pubmed |
| pubmed-article:3571289 | pubmed:author | pubmed-author:KaplanB EBE | lld:pubmed |
| pubmed-article:3571289 | pubmed:author | pubmed-author:RandallS KSK | lld:pubmed |
| pubmed-article:3571289 | pubmed:author | pubmed-author:EritjaRR | lld:pubmed |
| pubmed-article:3571289 | pubmed:author | pubmed-author:PetruskaJJ | lld:pubmed |
| pubmed-article:3571289 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3571289 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3571289 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:3571289 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3571289 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3571289 | pubmed:pagination | 6864-70 | lld:pubmed |
| pubmed-article:3571289 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3571289 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3571289 | pubmed:articleTitle | Nucleotide insertion kinetics opposite abasic lesions in DNA. | lld:pubmed |
| pubmed-article:3571289 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3571289 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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