Linked Life Data

3571241

Source:http://linkedlifedata.com/resource/pubmed/id/3571241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1987-6-5
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M15962, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16127, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16128, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16129, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16130, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16131, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16132, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16133, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16134, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16135, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16136, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16137, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16138, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16139, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16140, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M16141
pubmed:abstractText
We have isolated cDNA clones and determined the gene structure of chicken ovoinhibitor, a seven domain Kazal serine proteinase inhibitor. Using RNA blot hybridization analysis, the gene was identified initially as a region 9-23 kilobases upstream of the gene for the related inhibitor ovomucoid. Ovoinhibitor RNA appears in oviduct and liver. cDNA clones were identified by screening an oviduct cDNA library with a nick-translated DNA restriction fragment which contained an exon of the gene. The mature protein sequence derived from a cDNa clone is in excellent agreement with that which we obtained from direct sequencing of purified ovoinhibitor. The protein-sequencing strategy is reported. The P1 amino acids of the Kazal domains are consistent with the known broad inhibitory specificity of ovoinhibitor. The gene is about 10.3 kilobases in length and consists of 16 exons. Each Kazal domain is encoded by two exons. Like ovomucoid, introns fall between the coding sequences of the ovoinhibitor domains, an arrangement which may have facilitated domain duplication. The intradomain intron occurs in an identical position in all of the ovoinhibitor and ovomucoid Kazal domains, suggesting that this intron was present in the primordial inhibitor gene. We discuss the location of the intradomain intron in relation to the known structure of four Kazal inhibitors and suggest a scheme for the evolution of the ovoinhibitor gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
262
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5899-907
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Ovoinhibitor introns specify functional domains as in the related and linked ovomucoid gene.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't