Linked Life Data

3570530

Source:http://linkedlifedata.com/resource/pubmed/id/3570530

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-6-10
pubmed:abstractText
Semen samples were obtained from 4 healthy males on two occasions. One of the samples was liquefied at room temperature for 20 min while the other was diluted immediately with buffer to suppress proteolysis. A pool of immunoextracted calcitonin from liquefied and diluted samples, respectively, was subjected to gel chromatography in a separate experiment. In lyophilized samples the majority of immunoreactive calcitonin (CT) had an approximate molecular weight (Mr) of 10 kilodaltons (Kd) and immunoreactive PDN-21 was also present in the fractions. In diluted ejaculates CT was composed of larger species as well as the 10 Kd form. PDN-21 was present in fractions containing the 10 Kd form but could not be detected in the peaks of larger molecular weight, probably because of lower sensitivity of the PDN-21 assay compared to the CT assay. Our finding of CT and PDN-21 in the same fractions after immunoextraction with CT antibodies suggests the presence of proforms of CT in human seminal fluid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0105-6263
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
341-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Large forms of immunoreactive calcitonin in human seminal fluid exhibit PDN-21 immunoreactivity.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't