Linked Life Data

3569772

Source:http://linkedlifedata.com/resource/pubmed/id/3569772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-6-15
pubmed:abstractText
Massive small intestinal resection in the rat results in both structural and functional changes in the residual small bowel. Sodium-dependent D-glucose transport was examined in brush-border membrane vesicles derived from the proximal small bowel mucosa of male Sprague-Dawley rats 2 and 6 wk after a 66% distal jejunoileal resection or jejunoileal transection. Kinetic characteristics for the low-affinity, high-capacity system and high-affinity, low-capacity system were defined with rapid filtration under conditions of a zero-trans, 100 mM cis-NaSCN gradient. Mucosal weight, protein, and deoxyribonucleic acid content were increased in the residual intestinal segment compared to transected controls and morphometric studies revealed increased villus and crypt heights as well as an increased mitotic index. Postresection mean kinetic parameters for D-glucose transport at 2 wk (low-affinity system: Km, 177.5 +/- 45.1 microM; Vmax, 3.73 +/- 0.99 nmol X mg protein-1 X min-1; and high-affinity system: Km, 6.2 +/- 1.9 microM; Vmax, 0.12 +/- 0.06 nmol X mg protein-1 X min-1) and 6 wk (low-affinity system: Km, 267.8 +/- 83.1 microM; Vmax, 0.06 +/- 0.01 nmol X mg protein-1 X min-1; and high-affinity system: Km, 6.5 +/- 1.1 microM; Vmax, 0.06 +/- 0.01 nmol X mg protein-1 X min-1) were similar to values post-transection at 2 wk (low-affinity system: Km, 280.4 +/- 53.7 microM; Vmax, 3.05 +/- 0.32 nmol X mg protein-1 X min-1; and high-affinity system: Km, 9.1 +/- 1.3 microM; Vmax, 0.17 +/- 0.01 nmol X mg protein-1 X min-1) and 6 wk (low-affinity system: Km, 271.7 +/- 17.5 microM; Vmax, 4.69 +/- 0.23 nmol X protein-1 X min-1; and high-affinity system: Km, 10.6 +/- 4.2 microM; Vmax, 0.16 +/- 0.09 nmol X mg protein-1 X min-1). These kinetic data suggest that the hyperplastic response in adapting proximal small bowel after distal resection is accompanied by a persistence of the membrane functional characteristics for both sodium-dependent D-glucose transport systems despite an altered pattern of enterocyte proliferation and differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0016-5085
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1987-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Sodium-dependent D-glucose transport in brush-border membrane vesicles after massive distal small bowel resection in the rat.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't