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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1987-5-1
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pubmed:abstractText |
We investigated metabolic utilization of exogenous (modelled after lung surfactant) phospholipids by granular pneumocytes in primary culture. Cells were incubated for 21, 65, and 140 min with [3H-methyl]dipalmitoylphosphatidylcholine (DPPC) containing liposomes prepared from synthetic lipids. Radioactivity in cellular phosphatidylcholine (PC) declined steadily to 50% of the total trypsin-resistant cell-associated radioactivity. The proportion of radioactivity increased with time in cytidine-5'-diphosphate-choline and phosphorylcholine, which suggested reutilization of choline for PC synthesis. Cells incubated with liposomes for 2 h revealed that of the total cell-associated radioactivity, 7% was in lamellar bodies and 10% in the microsomal fraction. The lipid-associated radioactivity was 24% in "soluble," 96% in lamellar bodies, and 92% in the microsomal fraction. Percent of total PC label recovered in disaturated PC of microsomal fractions decreased (slope = -5.27%/h) with time of incubation (r = 0.67). Incubation of cells with liposomes containing ([3H-methyl]choline-[14C]palmitoyl) DPPC led to altered isotope ratios in both lamellar bodies and microsomes. These observations indicate that granular pneumocytes degrade exogenous PC and resynthesize PC from degradation products.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-1254572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-13671378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-217362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-2997104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-34631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-4068770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-4606365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-4613380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-5686279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-5800046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-580614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-583041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-588582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6256357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6411126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6546884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6687593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6688926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6696739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6746745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6893834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6894250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-6895081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-7165723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-7353276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-7440289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-932560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3558819-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1133-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3558819-1,2-Dipalmitoylphosphatidylcholine,
pubmed-meshheading:3558819-Ammonium Chloride,
pubmed-meshheading:3558819-Animals,
pubmed-meshheading:3558819-Chloroquine,
pubmed-meshheading:3558819-Choline,
pubmed-meshheading:3558819-Liposomes,
pubmed-meshheading:3558819-Lung,
pubmed-meshheading:3558819-Methylamines,
pubmed-meshheading:3558819-Organoids,
pubmed-meshheading:3558819-Pulmonary Surfactants,
pubmed-meshheading:3558819-Quinacrine,
pubmed-meshheading:3558819-Rats
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pubmed:year |
1987
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pubmed:articleTitle |
Degradation of dipalmitoyl phosphatidylcholine by isolated rat granular pneumocytes and reutilization for surfactant synthesis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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