| pubmed-article:355847 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:355847 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:355847 | lifeskim:mentions | umls-concept:C0085472 | lld:lifeskim |
| pubmed-article:355847 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:355847 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:355847 | pubmed:dateCreated | 1978-10-25 | lld:pubmed |
| pubmed-article:355847 | pubmed:abstractText | The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells. | lld:pubmed |
| pubmed-article:355847 | pubmed:language | eng | lld:pubmed |
| pubmed-article:355847 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:355847 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:355847 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:355847 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:355847 | pubmed:issn | 0026-8925 | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:SchellJJ | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:HolstersMM | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:DepickerAA | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:MessensEE | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:van MontaguMM | lld:pubmed |
| pubmed-article:355847 | pubmed:author | pubmed-author:de WaeleDD | lld:pubmed |
| pubmed-article:355847 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:355847 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:355847 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:355847 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:355847 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:355847 | pubmed:pagination | 181-7 | lld:pubmed |
| pubmed-article:355847 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:355847 | pubmed:meshHeading | pubmed-meshheading:355847-B... | lld:pubmed |
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| pubmed-article:355847 | pubmed:meshHeading | pubmed-meshheading:355847-T... | lld:pubmed |
| pubmed-article:355847 | pubmed:year | 1978 | lld:pubmed |
| pubmed-article:355847 | pubmed:articleTitle | Transfection and transformation of Agrobacterium tumefaciens. | lld:pubmed |
| pubmed-article:355847 | pubmed:publicationType | Journal Article | lld:pubmed |
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