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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1979-7-16
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pubmed:abstractText |
A rapid, high-yield method for purification of 6-phosphogluconate dehydrogenase from Escherichia coli K-12 is described. Sonic extracts prepared from heat-induced cultures of strain RW184, doubly lysogenic for the specialized transducing bacteriophage lambdacI857St68h80dgndhis and bearing a deletion of the gene for glucose 6-phosphate dehydrogenase, contained levels of 6-phosphogluconate dehydrogenase 15- to 20-fold higher than cultures of wild-type cells. Affinity chromatography on blue dextran-Sepharose with batchwise elution with 1 mM nicotinamide adenine dinucleotide phosphate affected a further 10-fold purification. Enzyme prepared in this manner was homogeneous according to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels and immunoelectrophoresis using antiserum directed against it. Fructose 1,6-diphosphate is an inhibitor of enzyme activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-13587488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-164664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-172907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-174104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4364067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4396792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4564298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4590471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4602166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-4923718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-5337843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-786365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/35519-791290
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Combined use of strain construction and affinity chromatography in the rapid, high-yield purification of 6-phosphogluconate dehydrogenase from Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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